Atomic absorption spectroscopy or AAS is defined as a spectro-analytical techniques used for determining quantities of chemical elements. Present in a sample. It uses the absorption of light or optical radiation by the free atoms in the gas state. This technique is used in analytical chemistry for determining the concentrated level of any particular analyte, associated with the sample to be analyzed. This technique is primarily used to determine more than 70 elements in solution state or in dissolved samples. Main applications are in pharmacology, toxicology, foods and environmental .
Understanding the importance of AAS:
Just like HPLC, it is important to understand operation and basics of AAS. It is the primary objective of any trace metal analyst. At present, this technique has showcased a new path of light. These analysts have now made inroads in various fields like oceanography, environmental monitoring, foods and even n pharmaceutical areas. The growing awareness of different environment concerns have already compelled the standard bodies to lower their limits on trace metals.
Importance of learning AAS:
As in GC, there are different advantages, which make AAS a great learning option, especially for the trace metal analyst. With the introduction of atoms in light path, the absorbed light amount also increases at a great rate. There are different advantages, which help AAS to be the best testing exam. You will receive high sample throughout and with easy to use interface. With this technique, you will receive high precision. Moreover, as this technique is considered to be an inexpensive one, you will obtain high analysis throughputs.
For the sample technique:
Graphite furnace is considered to be advanced and most commonly used sensitivity sampling technology. The primary aim of this furnace in AAS deals with high solid samples and slurries, which can be analyzed, along with soluble samples. This graphite furnace also requires smaller sample quantities, which are mainly 5-60 ul. There is a slight difference in atomization procedure, too. however, there are some disadvantages associated with graphite furnace as well, like it is an expensive technique and needs help of experienced operators only.
Flame method of AAS:
AAS flame method has been an age old established analysis techniques. AAS has now been used in various labs for more than 40 years now. Through this method, nearly 50 metals and other forms of metalloids can be easily analyzed down to trace range in quantitative manner. The products are extremely selective and also known for their detection sensitivity characteristic.
Taking help of flames:
Solely depending on the analytical element, there are different types of flame temperature used for AAS technique. The most commonly used example is acetylene or air flame, where the temperature is between 2200 to 2400 degree Celsius. And nitrous oxide flames are used.